Veterinaria

Research article

Molecular Diagnosis of Bovine anaplasmosis in District Lahore

Aqsa Mushtaq a, Aneela Zameer Durrani b, Amara Mushtaq a, Shahida Parveen a, Waheeda Raana a, Rehan Rafique a, Tamoor Azeem c, Sajid Umar* c

a Veterinary Research Institute (VRI), Lahore.

b Department of Clinical Medicine, University of Veterinary & Animals Sciences, Lahore.

c *Department of Pathobiology, Faculty of Veterinary & Animals Sciences, PMAS-Arid Agriculture University Rawalpindi.

Abstract

Bovine anaplasmosis is a tick borne rickettsial disease wide spread in tropical and subtropical areas. A comparative study was conducted to check the prevalence of bovine anaplasmosis in different age groups of cattle and buffaloes. A total of 160 samples were collected (80from each) during May, June, July, August. Screening was done by blood smears, stained by Giemsa'wright staining technique and later the blood samples from the same animals were also processed by PCR. On the basis of Microscopic examination overall 11.25% (18\160) prevalence was recorded. On the basis of polymerase chain reaction (PCR) prevalence of Anaplasma marginale 25.6% (41\160) was recorded, showing the presence of carrier animals in District Lahore. The Polymerase chain reaction showed that the prevalence of Bovine anaplasmosis is more in cattle 32.5% (26\80) than in buffalo18.75 % (15\80). The results have demonstrated the high efficacy of polymerase chain reaction assay as compare to microscopic examination.

Keywords: Anaplasmamarginale, PCR, Buffalo, Cattle, Prevalence.

Received 13 October, 2014; Revised 05 January, 2015; Accepted 17 January, 2015

*Corresponding author: Sajid Umar; Email: sajncvi@gmail.com