Veterinaria

Research paper

Rapid Detection of Foot and Mouth Disease Virus (FMDV) through Co-agglutination Test

Baitullah Khan a*, Rafiullah Khan b, Khawar Ali Shahzad c, d

a Veterinary Research and Disease Investigation Centre, Hangu Road Kohat, 26000, Pakistan

b Department of Poultry Sciences, Agriculture University Peshawar, 25000, Pakistan

c Department of Microbiology and Immunology, Southeast University, Nanjing, 210009, China

d Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, 54000, Pakistan

Abstract

In present study, a Coagglutination test (COAT) was optimized for rapid detection of Foot and Mouth Disease Virus (FMDV). Immune sera against FMDV O, A and Asia1 strains were raised separately and in combination. Protein A, containing Staphylococcus aureus pellet was sensitized with rabbit anti-FMD sera. COAT reagents were tested against laboratory FMD virus. The 1/100 dilution S. aureus with 250µl serum was found specific for FMD virus. COAT reagents depicted no agglutination with phosphate buffer saline, cell culture media, animal saliva and PPR virus. Forty vesicular fluid samples were tested with combined conjugate (prepared by using trivalent sera), in which 38 were found positive. Of 38 vesicular fluid (VF) samples tested, 33 of them were O, 3 were Asia1 and 2 were A strains of FMDV. COAT was found 100% sensitive and highly specific for detection of FMD virus in vesicular fluid. As this test was easy to perform it could be used for rapid diagnosis of FMD virus.

Keywords: Coagglutination test, FMDV, Asia 1, Agglutination, Trivalent sera

Received: October 10, 2014 Revised: February 16, 2014 Accepted: March 4, 2015

*Corresponding author: Baitullah Khan Email: bait.living@gmail.com