Comparative Efficacy of Metronidazole in Combination Colistinsulphate and Probiotics against Clostridium perfringens and Their Effects on Histopathological Features of Visceral Organs in Broiler
Sidra Aslam a, Aneela Zameer Durrani b, Muhammad Rizwan c*, Muhammad Kashif d
a Department of Livestock & Dairy Development, Punjab-Pakistan
b Department of Clinical Medicine & Surgery, University of Veterinary & Animal Sciences-Lahore, Pakistan
c Muhammad Nawaz Sharif University of Agriculture-Multan, Pakistan
d College of Veterinary & Animal Sciences, Jhang, Pakistan
Necrotic enteritis is a widespread and economically disturbing bacterial disease in modern broiler flocks. The primary etiologic agent of necrotic enteritis is Clostridium perfringens type A. The current study was designed to determine the histopathological changes in broilers challenged by C. perfringens and determine the comparative efficacy of colistinesulphate, metronidazole and probiotics on histopathological changes. A total of 100 birds were divided into 5 groups (A, B, C, D and E) each having 20 birds. Each group was treated according to its trial. Birds in all groups (except group E) were inoculated orally with Clostridium Perfringens (3x1010 CFU/ml/bird) on day 9th, 10th, 11th, 12th, 13th of chick’s age twice a day. Chicks in group A were administered probiotics 1ml/L in drinking water for the first seven days and 2ml/L for rest of the 35 days. Chicks in Group B & C were administered ColistinSulphate2 g/1 lit drinking water and Metronidazole Syrup 4 ml/1 lit drinking water after two days of inoculation for five continuous days. Group D and E represented positive and negative control groups respectively. Birds were observed twice a day for clinical signs and mortality. Weight of birds was measured at 20th, 25th, 30th and 35th day. Most sensitive antibiotic was found metronidazole as it gave 95 % efficacy on day 5th of the treatment while cholistinesulphate gave 70 % recovery on the same day and probiotic gave only 50 % recovery. Four birds from each group were slaughtered at 20th, 25th, 30th and 35th day for necropsy to determine score of gross pathological lesions and sample collection (Liver, Kidney and Intestine) for histopathological studies. The results had significant difference among different groups on different days. Findings of the study revealed that metronidazol effectively abolish the adverse effects of C. perfringens. Use of Antibiotics especially metronidazole along with probiotics can be useful in the treatment and control of C. perfringens infections in poultry birds. Moreover, metronidazole is better choice for the treatment and control of clostridial infections in poultry birds as compared to Colistinsulpahate.