Biomedical Letters

Isolation and Antibiotic Susceptibility of Gram Negative Bacteria Associated with Urinary Tract Infection

Waqas Ahmed 1, Muhammad Ashraf 1, Adeel Sattar 2, Sajjad-ur-rehman 1, Muhammad Abu Bakr Shabbir 1, Nabeel Ijaz 3, Rais Ahmad 1, Saiqa Iqbal Rao 4, Farzana Shahin 4

1 Institute of Microbiology, University of Agriculture, Faisalabad, 38000, Pakistan

2 Department of pathology, University of Agriculture, Faisalabad, 38000, Pakistan

3 Department of Theriogenology, University of Agriculture, Faisalabad, 38000, Pakistan

4 University of Veterinary and Animal Sciences, Lahore 54000, Pakistan

Abstract

Urinary tract infections (UTI) are most commonly observed infections in clinical practice Caused by Escherichia coli and other Gram negative bacteria. The present study was conducted to isolate Gram negative bacteria from UTI and non-UTI persons within different age groups. A total of 150 urine samples were collected from UTI patients and non-UTI patients. Urine sample were inoculated on selective media for the isolation of E. coli, Klebsiella, Proteus and Pseudomonas spp. The isolated Gram negative bacteria were identified on the basis of culture characteristics, morphology and biochemical characteristics. Antimicrobial susceptibility testing was done on the Muller-Hinton agar by disc diffusion method. Associated risk factors with urinary tract infection were determined. The results showed that E. coli were 80.77%, Klebsiella 10.25%, Pseudomonas 6.4% and Proteus 2.5% in UTI patients. In this study urinary tract infection was more in females as compared to males. UTI were found more in 18-45 year old patients. The overall results showed that E. coli was the major pathogen found in these infections. The results of antibiotic susceptibility testing showed that E. coli isolates were resistant to ampicillin and tetracycline, moderately susceptible to Nitrofurantion and Nalidixic acid and Susceptible to Ciprofloxacin and Enoxacin. Among associated factors, stone formation was found in 22% and enlarged prostate gland in 8% cases.

Keywords: Urinary tract infection, Escherichia coli, antibiotic resistance.

Received April 29, 2015 Revised May 11, 2015 Accepted May 15, 2015

*Correspondence: Waqas Ahmed Email: waqaschatha@yahoo.com Phone: +923338072087.