Biomedical Letters

Biochemical changes in saliva and blood of diabetic patients

Kalsoom Zaigham 1, Khawar Ali Shahzad 1, 2, Abdul Razzaq 1, Muhammad Moeez 1, Muhammad Zaigham Javed 1

1 Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000, Lahore, Pakistan

2 Department of Microbiology and Immunology, Southeast University, Nanjing 210009, China

Abstract

Owing to the contribution of serum derived components into whole saliva. It is hypothesized that the changes in serum composition caused by diabetes would be reflected in whole saliva. The present study was therefore aimed to evaluate the biochemical changes in blood and saliva of diabetic patients. For this purpose total 50 blood samples were collected (25 from diabetic patients and 25 from healthy individuals) from Jinnah Hospital Lahore, Pakistan. Total 50 samples of saliva were also collected with the same ratio. The serum from the blood samples was separated by centrifugation at 3000 rpm for 10 minutes. Serum and saliva was then analyzed for biochemical changes viz estimation of serum calcium, phosphate, total protein, alkaline phosphatase and salivary calcium, phosphate and amylase. Outcomes revealed that in diabetic parent's metabolism of minerals (calcium and phosphate) get altered significantly. In diabetic condition, phosphate decreased in blood and saliva with reduction in amylase levels while other biochemical changes were found insignificant. It was suggested that saliva tests can be used to evaluate the diabetes and oral health in diabetic patients.

Keywords: Diabetes, alkaline phosphatase, calcium, phosphate, amylase, oral health.

Received January 01, 2016 Revised February 10, 2016 Accepted February 23, 2016

*Correspondence: Kalsoom Zaigham Email : kalsimbb@yahoo.com Contact: +92 3117502868