Science Letters

Effect of different media compositions and physicochemical conditions on the in vitro growth of Pasteurella multocida

Farzana Shahin*, Mudasser Habib, Muhammad Salahuddin Shah, Noubahar Hussain, Shahnaz Adeeb Khanum

Nuclear Institute for Agriculture and Biology (NIAB), Jhang Road, Faisalabad, Pakistan

Abstract

Hemorrhagic septicemia (HS) is an acute disease of cattle and water buffaloes. In Pakistan, it is frequently caused by B: 2 serotype of Pasteurella multocida. Vaccination is the principal mean of prevention. In vaccine manufacturing, higher yields of bacterial cell mass are of great value in terms of process economics. Present work extends the optimization of various physicochemical conditions of culture, e.g., effect of different media compositions, pH and inoculum size. Casein-sucrose-yeast media (CSY) was prepared with different concentrations of yeast extract, casein hydrolysate, and sucrose with different inoculums sizes. The maximum growth of P. multocida was achieved by using 0.6% sucrose and 8% inoculum size in CSY medium after 16 h incubation (OD 1.73) and at the pH range of 7.0-8.0. It was also found that different concentrations of media components and physical conditions have significant impact on the growth kinetics of P. multocida.

Keywords: Hemorrhagic septicemia; Pasteurella multocida; physicochemical conditions; bacterial cell mass.

Received March 12, 2014; Revised May 02, 2014; Accepted May 12, 2014

Corresponding author: Weimin Fan; Email: f.shaheen64@yahoo.com