Science Letters

Characterization of the newly isolated antimicrobial strain Streptomyces goshikiensis YCXU

Muhammad Faheem 1, Waseem Raza 2*, Zhao Jun 1, Sadaf Shabbir 1, Nasrin Sultana 1

1 Institute of Soil Science, Chinese Academy of Sciences, Nanjing, PR China

2 College of Resource and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China

Abstract

A rhizosphere bacterial strain coded as Streptomyces goshikiensis YCXU with broad spectrum antifungal activity was isolated from a cucumber field infested with Fusarium oxysporum f. sp. niveum. The strain YCXU showed antagonism to a broad range of phyto-pathogenic fungi and bacteria as well as strain YCXU produced volatile organic compounds that could also reduce fungal growth up to 40% as compared to control, concluding that it can be used as biocontrol agent. Because of little information about the newly isolated strain, we further characterized the S. goshikiensis YCXU. The strain YCXU showed maximum growth on glucose containing yeast-malt extract (YME) medium at pH 7 and pink spores were produced after 7 days of incubation at 28�C. The strain YCXU exhibited nitrate and melanin production, showed blood hemolysis, and hydrolyzed the casein, gelatin, starch, tyrosine, and hypoxanthine. This characterization will aid further research regarding the strain S. goshikiensis.

Keywords: Characterization, growth, hydrolysis, Streptomyces goshikiensis YCXU.

Received April 06, 2015 Revised May 29, 2015 Published online first June 30, 2015

*Corresponding author: Waseem Raza Email: waseem@njau.edu.cn