Biomedical Letters

Correlation of renal ischemia with oxidative stress and generation of reactive nitrogen species

Kalsoom Zaigham 1, Lutfullah1, Khawar Ali Shahzad 1, 2, Muhammad Shahzad Iqbal 3, Muhammad Zaigham Javed 1

1 Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000, Pakistan.

2 Department of Microbiology and Immunology, Southeast University, Nanjing 210009, China.

3 Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, Pakistan.

Abstract

Reactive oxygen species (ROS) and nitric oxide (NO) are important mediators of tissue injury and renal ischemia (RI). The study was aimed to investigate the correlation of oxidative stress with RI. Blood samples were collected from 25 RI patients and 25 normal individuals. Plasma urea, uric acid and creatinine levels were monitored as markers of renal injury. Glutathione oxidase, Superoxide dismutase (SOD), catalase and malondialdehyde (MDA) were used as markers of oxidant stress. NO was used as a biomarker of reactive nitrogen species (RNS) formation. SOD, MDA, give complete name of this first, catalase and NO were assessed by spectrophotometric assay while renal parameters were estimated by enzymatic kits. Significant increase in plasma creatinine, urea and uric acid were found showing renal injury. Significant decrease in catalase, SOD and GSH with compare able increases in MDA and NO were observed. The data was evaluated statistically by using t-test according to which overall significant difference was observed in oxidative stress indices, reactive nitrogen species and renal indices in case group of renal ischemia as compared to control group as p<0.05. Negative correlation was observed in oxidative stress parameters with NO and renal parameters except for MDA with positive correlation. Oxidative stress and RNS generation occur in the kidney during ischemia.

Keywords: Renal ischemia (RI) Oxidative stress, Reactive oxygen species, Nitric Oxide

Received February 23, 2015 Revised April 02, 2015 Accepted April 12, 2015

*Correspondence: Kalsoom Zaigham Email: kalsimbb@yahoo.com Contact +923117502868