Biomedical Letters

Isolation and identification of Methicillin and Vancomycin Resistance Staphylococcus aureus From Pus Samples of Injured Skin Patients in Lahore, Pakistan

Wasif Ghias 1, Musarrat Sharif 1*, Maham Yazdani 1, Farheen Ansari 1, Masood Rabbani 2

1 Institute of Molecular Biology and Biotechnology, The University of Lahore, Defense Road, Lahore, Pakistan

2 University Diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan

Abstract

Staphylococcus aureus especially methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Staphylococcus aureus (VRSA) is a major health problem recognized as the most important nosocomial pathogen often causing respiratory tract infection, gastrointestinal infection, urinary tract infection, surgical site infection, blood stream infection and soft tissue infection etc. This study was designed to identify MRSA and VRSA from the pus of injured skin of hospitalized patients. A total of 200 pus samples were collected from four hospitals of Lahore city i.e. Mayo Hospital, Services Hospital, Ganga ram Hospital and Jinnah Hospital. Staphylococcus aureus were isolated from samples by culturing on nutrient agar, mannitol salt agar and identified by morphological characteristics on selective media and biochemical characterization. For MRSA screening, 1µg oxacillin and 30µg cefoxitin discs were used. Resistance isolates was screened by vancomycin agar screening method and confirmation of resistance to vancomycin was done by measuring MIC. It was concluded that hygiene is not sufficiently adequate. To eliminate MRSA and MRSA complete surface sanitation and hand hygiene are necessary.

Keywords: Nosocomial infection, antibiotic, resistant, MRSA, VRSA.

Received October 24, 2016 Revised November 20, 2016 Accepted December 13, 2016

*Correspondence: Musarrat Sharif Email musarratsharif388@gmail.com Contact: +92-3104008714