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Biomedical Letters ISSN 2410-955X
2018 | Volume 4 | Issue 1 | Pages 1-14
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Review article
Pichia pastoris expression system: a potential candidate to express protein in industrial and biopharmaceutical domains
Imran Safder 1, Sajad Khan 5, Iram-us Islam 2, Muhammad Kazim Ali 2,4, Zainab Bibi 2,4, Muhammad Waqas 2, 3*

1 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-72570, Pakistan
2 Department of Biotechnology, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal campus, Karachi, Pakistan
Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China
4 Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi-72570, Pakistan
5 Dadabhoy Institute of Higher Education, Karachi, Pakistan

Abstract
The methylotrophic yeast Pichia pastoris is a widely used, well established protein expression system for the production of biopharmaceutical and industrial enzymes. It is by far one of the most commonly utilized microbial species due to its high cell density growth, ease to use, rapid expression time with low cost and post-translation modification system. Moreover, its strong regulated promoters, the capacity to meet high production yield even of proteins of large molecular weight, simple recovery and purification steps makes it superior in contrast to other expression systems. Recent advancements in high-throughput genomics and development of humanized glycosylated Pichia pastoris strain will increase its importance in biopharmaceutical production. This review aims to highlight Pichia pastoris expression system as a potential candidate to express industrial and biopharmaceutical proteins alongside bioprocess design and expression optimization strategies to increase product yield. 

A R T I CL E  I N F O

Received
November 27, 2017
Revised
January 07, 2018
Accepted
January 19, 2018

*Corresponding author
Muhammad Waqas
E-mail
waqasmt@hotmail.com

Keywords
Yeast
Pichia pastoris
Recombinant protein expression
Bioprocess design
Industrial applications