An Innovative Approach to Treat Brucellosis in Buffaloes
Haisem Ur Rasool 1, Muhammad Avais 1, Masood Rabbani 2, Rais Ahmed *2, Khushi Muhammad 2, Muhammad Asad Ali 2
1 Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore-54000, Pakistan.
2 Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore-54000, Pakistan.
Brucellosis in cattle and buffaloes is caused by Brucella abortus and characterized by late term abortions, placentitis, retained placenta, stillbirth, orchitis, and infertility. The experimental study included healthy (A and B groups) and brucellosis positive (C and D groups) buffaloes. The animals in both the groups A and C were given 1) four injections of long acting oxytetracycline (20mg/kg body weight; BW) intramuscularly (IM) repeated after every 48 hours, 2) seven consecutive injections of streptomycin (13mg/kg BW IM), 3) three consecutive injections of flunixin meglumin (2mg/kg BW IM) for first three days of the treatment followed by three injections (IM) of Selevit for next 3 consecutive days and 4) combined vaccine on 10th day of the treatment. Each animal of group B and D served as negative and positive control, respectively. Each animal of the group C and D were positive while animals of the group A and B negative to ELISA on six months post-treatment and PCR on subsequent parturition. However, calves of group C, A and B were negative while calves of group D were positive to ELISA and PCR. In conclusion, chemo-immunotherapy is an effective way to treat Brucella infected buffaloes in local circumstances.