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Biomedical Letters ISSN 2410-955X
2018 | Volume 4 | Issue 2 | Pages 40-46
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Research article
Association of Pathogenicity Genes (cvaC, iss, iutA, Stx1A, Stx2A and Vat) of E. coli with Gross and Histopathological Lesions of Colibacillosis in Broilers
Hina Sharif 1, Muhammad Tariq Javed 1*, Hammad Ghafoor 7, Muhammad Younis 8, Saad Ullah Khan 9, Aziz ur Rehman 1, Khurram Ashfaq 2, Gulbina Saleem 3, Farkhanda Manzoor 4, Narmeen Tariq 5, Asim Rafique 6

Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Faisalabad, Pakistan 
Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture Faisalabad, Faisalabad, Pakistan
Department of Pathology, University of Veterinary and Animal Science, Lahore, Pakistan
Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Institute of Microbiology, University of Agriculture Faisalabad, Pakistan
Department of Poultry Production, Government of Punjab, Faisalabad, Pakistan 
Department of Microbiology and Immunology, Southeast University Medical School, Nanjing, China
8 Institute of Life Sciences, Southeast University, Nanjing 210000, China
Department of Clinical Medicine and Surgery, College of Veterinary and Animal Science, Jhang, Pakistan

Abstract
Colibacillosis is an important disease of poultry caused by avian pathogenic Escherichia coli (APEC). The E. coli infection is associated with complications in chicken, which results in significant economic losses. For this purpose, study was conducted in the Faisalabad region of Pakistan. Study included the visits to the nearby poultry farms suspected of E. coli infections. The sampling was done from the farms and various diagnostic laboratories across Faisalabad region, including diagnostic laboratory University of Agriculture Faisalabad, Pakistan and district diagnostic laboratory. The main purpose of the study was to detect different pathogenicity genes (iutA, iss, cvaC, stx1, stx2 and vat) and associated lesions in broilers. Morbid organs including heart, liver, spleen, intestine, etc. of clinically positive birds were collected for histopathological studies and culture isolation. For histopathological study, tissues were preserved in 10% formalin. Culturing was done on MacConkey agar and the positive growth was confirmed on triple sugar iron agar. DNA was extracted from bacterial colonies. The gene was detected on different sample infected with E. coli through PCR. The results concluded that the prevalence of iutA genes was detected @ 96%, iss 88%, cvaC 64%, stx1 and stx2 @ 0%, while vat was detected 40% on different organs of broilers, respectively. 

A R T I CL E  I N F O

Received
August 27, 2018
Revised
October 19, 2018
Accepted
October 27, 2018

*Corresponding Author
Muhammad Tariq Javed
E-mail
javedmt@gmail.com

Keywords
Escherichia coli
Pathogenicity
Colibacillosis
Broilers