Biomedical Letters

Whole cell protein profiling of Brucella abortus strain 19 through SDS-Page technique

Sajida Mushtaq 1*, Adeela Sharif 2, Asghar Ali 3

1 PMAS- Arid Agriculture University, Rawalpindi- 46000, Pakistan

2 PARC Institute of Advanced Studies in Agriculture, Islamabad-44000, Pakistan

3 Fatima Memorial Hospital (FMH) College of Medicine and Dentistry, Lahore, Pakistan

Abstract

Brucellosis is an important zoonotic disease which cause direct and indirect economic losses such as reduction in meat and milk production and abortion in mammals. Brucella abortus is the causative agent of bovine brucellosis. Usually, B. abortus vaccines; Strain 19 and RB51 are used as part of an eradication program or can be used to control the disease in endemic areas. Routine vaccination is often done in calves to minimize the production of persistent antibodies that can interfere with serological tests. Therefore, the present study was designed to determine the whole cell protein profiling of B. abortus strain 19 and to compare the vaccinal strain and field isolates on the basis of protein patterns to observe the extent of homology between them through SDS-PAGE. Pure culture of B. abortus was subjected to centrifugation followed by sonication and washing by normal saline. The proteins released after sonication were separated out by centrifugation. The sonicated supernatant was used for SDS-PAGE analysis. Our findings revealed that, a single vaccine against all the strains can be used to overcome brucellosis in cattle. However, for the development of specific vaccine against specific isolate needs further studies by using SDS-PAGE.