Biomedical Letters

Plant growth regulators for efficient in vitro regeneration of Solanum lycopersicon

Amin ur Rehman 1, Sania Nisar 2*, Baseer Ahmad 3, Abdul Basit 1, Muhammad Aizaz 1, Muhammad Shafiq Ahmad 4, Muhammad Tariq Javeed 4, Naveed Sabir Butt 1, Quratulain Hanif 5, 6*

1 College of Biological Sciences, Centre for Life Sciences, China Agricultural University, Beijing, China

2 Department of Genetics, Hazara University Mansehra, KPK, Pakistan

3 College of Animal Sciences and Technology, China Agricultural University, Beijing, China

4 College of Agronomy and Biotechnology, China Agricultural University, Beijing, China

5 National Institute for Biotechnology and Genetics Engineering, Faisalabad, 577, Pakistan

6 Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan

Abstract

Leaf, apical meristem and nodal explants of Solanum lycopersicon were cultured in many permutations and concentrations of BAP and IBA to improve in vitro environments and to detect the reaction of tomato cultivar. All the explants always formed calli with the diversity of color and textures after three weeks. Callus induction was observed at a low concentration of hormonal arrangement in case of leaf explant; however, high concentration was effective in case of apical meristem and nodal explants. No early morphogenic response was detected, after 6 weeks morphogenic response of callus was perceived at 1.2 mg/L BAP + 2 mg/L IBA and clear shoot primordia were formed by nearly all explants. Calli consuming shoot embryonic were transferred to hormones-free MS media for additional differentiation and growth. Whole redevelopment i.e. Shoot and root development were detected after 8 weeks. A regular of 10 shoots with insufficient roots was detected on BAP+IBA (1.2 mg/L+2 mg/L) from calli of leaf explants. While 7-8 shoots with numerous roots and 5 shoots with several roots in case of apical meristem and nodal callus individually.