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ISSN 2410-955X - An International Biannual Journal
In silico structural insight and functional evaluation to predict novel non-coding RNAs of Enterobacter cloacae complex
Abbeha Malik, Muhammad Nasir Iqbal*
Department of Bioinformatics, Institute of Biochemistry, Biotechnology and Bioinformatics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract
The non-coding RNA (ncRNA) produces functional RNA molecules instead of encoding proteins, however, the ncRNAs contain information to perform the function. Most genetic information is encoded by proteins while most of the genetic information of mammals and other complex organisms is transcribed into ncRNAs. The current study was designed to predict the ncRNAs in the genome of the Enterobacter cloacae complex by employing in silico approaches. Various putative ncRNAs were predicted in four different species of Enterobacter cloacae complex. Extensive in silico analyses were performed and specific promoters were predicted for all the selected ncRNAs. The predicted promoter regions were validated for further analyses. The selected ncRNA was utilized for secondary structure prediction. All the predicted secondary structures were validated through various evaluation tools and secondary structures were observed suitable. All the selected ncRNAs were observed stable and characterized based on hairpin loops, least MFE value and promoter regions. In conclusion, the predicted ncRNAs have the ability to perform stable functions.
A R T I C L E I N F O
Received
June 12, 2022
Revised
July 28, 2022
Accepted
September 02, 2022
*Corresponding Author
Muhammad Nasir Iqbal
E-mail
nasir.iqbal@iub.edu.pk
Keywords
Non-coding RNAs
Transfer RNAs
Ribosomal RNAs
micro RNAs
intergenic region
minimum free energy
2022 | Volume 8 | Issue 2