Isolation, identification, and antibiotic sensitivity patterns of bacterial strains isolated from human pus samples

Research article | https://doi.org/10.47262/BL/11.1.20250313

Shabana Ramzan 1, Khawar Ali Shahzad 1, 2*, Muhammad Faizan Munawer 1, Munazzah Marryum 1, Mavra Saleem 1, Farwa Bashir 3

1Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan

2Shanghai Fourth People's Hospital, and School of Medicine, Tongji University, Shanghai, 200120, China

3Department of Zoology, The Government Sadiq College Women University, Bahawalpur, Pakistan

Abstract

The objective of this study was to isolate, identify, and evaluate the antibiotic sensitivity patterns of bacterial strains in order to cure the infections in patients with wounds S. aureus, Enterobacter, Citrobactor and E. coli, Klebsiella spp. were isolated from pus and wound samples, and they were identified based on morphology, colony, gram staining and various biochemical tests. A total of 128 samples were collected from pus and wound infections. Bacterial growth was observed in 65 of these samples. Data showed a higher infection rate among males (53%) compared to females (47%). The identified bacteria include Escherichia coli (E. coli) (32%), Staphylococcus aureus (S. aureus) (23%), and Pseudomonas species (23%), being the most prevalent pathogens. Gram-negative bacteria were found to be more prevalent among the isolated pathogens. The most frequently identified bacteria included Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and various species of Pseudomonas showed high susceptibility to vancomycin and imipenem but lower susceptibility to ampicillin and tetracycline. Gram-negative bacteria showed higher sensitivity to antibiotics such as ciprofloxacin, vancomycin, ampicillin, imipenem, and levofloxacin. Our study successfully isolated and identified bacterial strains from human pus samples. Antibiotic sensitivity patterns revealed valuable information for effective treatment strategies.