Copyright 2024 The Science Publishers
All Rights Reserved for Website Design.
.png){kind=small}
ISSN 2410-955X - An International Biannual Journal
Molecular analysis of tetracycline resistant gene in gram-negative bacteria isolated from dairy farms
Yasir Ali 1, Qurat-ul-Ain Hira 2, Waqas Khan 3, Tanveer Hussain 4, Ayesha Maqbool 4
1Department of Biology, Virtual University of Pakistan
2Department of Biotechnology, Virtual University of Pakistan
3Department of Biotechnology, University of Sargodha, Punjab, Pakistan
4Department of Molecular Biology, Virtual University of Pakistan
Abstract
The present study was conducted from April 2019 to June 2019 in order to detect the tetracycline A resistant Gene in gram negative bacteria. A total of 40 buffalo's milk samples were collected randomly by aseptic technique, brought to laboratory. They were inoculated on Blood and MacConkey agars and then incubated at 37°C for 24 hours whereby growth of colonies were further confirmed with catalase test, Coagulase test, Oxidase test, Gram staining and API 20 E kit. Bacterial DNA was isolated using the boiling method. The Tet A gene (210 bp) was amplified in thermal cycler and run on 1.8-gram agarose gel with 50 kb ladder. The most predominant bacterial colonies observed were of Escherichia coli (10 (33.3%) followed by Klebsiella pneumonia 5 (16.7%), Klebsiella spp. 5 (16.7 %), Pseudomonas spp. 10 (33.3%) and prevalence of tetracycline A gene was 8 (26.7%).
A R T I C L E I N F O
Received
June 24, 2020
Revised
August 12, 2020
Accepted
September 17, 2020
*Corresponding Author
Ayesha Maqbool
E-mail
ayesha.maqbool@vu.edu.pk
Keywords
Tetracycline-resistant
Gram-Negative bacteria
Milk
Polymerase chain reaction
Case report | https://doi.org/10.47262/BL/6.2.20200724
2021 | Volume 6 | Issue 2