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ISSN 2410-955X - An International Biannual Journal
Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria
Nighat Nawaz 1, Irshad Ahmad 2*, Simon G. Patching 3, Iqbal Hussain 1*

1 Department of Chemistry, Islamia College Peshawar, Peshawar, Pakistan
2 Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan
3 School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK

Aqueous extract of the plant Tribulus terrestris was used to reduce chloroauric acid for synthesizing gold nanoparticles (AuNPs). In this green synthesis, the reaction proceeded to give a red/purple color that was monitored by UV-vis spectrophotometry, where the formed AuNPs had an absorption band with  max of 550 nm. In terms of the highest absorbance at 550 nm, reaction conditions were optimized at a temperature of 75°C, at pH 7 and using a reaction time of 4 h. The integrity of the synthesized AuNPs was confirmed and their physical properties were characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM), which also showed evidence that plant metabolites contributed to capping or stabilization of the AuNPs. XRD spectra suggested a particle size of around 40 nm and SEM images revealed spherical and relatively uniform and disperse particles with a size of less than 0.1  m. In a broth microdilution assay, the AuNPs showed inhibitory effects against Gram-negative Escherichia coli, but not against Gram-positive Enterococcus faecalis. The AuNPs showed no hemagglutination activity or cytotoxic activity against human blood cells, which is important for them to be explored as therapeutic antibacterial agents.


A R T I C L E  I N F O

July 16, 2021
August 19, 2021
September 21, 2021

*Corresponding Author
Iqbal Hussain
Irshad Ahmad

Antibacterial activity
Gold nanoparticles
Green synthesis
Plant extract
Tribulus terrestris

2021 | Volume 7 | Issue 2