Biomedical Letters

Anti-microbial, antioxidant potential of Cassia fistula and study their phytochemical assessments

Musarrat Sharif 1*, Farheen Ansari 1, Najam Ul Hassan 2

1 Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan

2 Department of Pharmacy, The University of Lahore, Lahore, Pakistan

Abstract

Cassia fistula (C. fistula) is a flowering plant and a member of Fabaceae family. This study was designed to examine antibacterial, antioxidant and phytochemical activity of ethanolic extract of C. fistula plant. The microbial inhibitory effect of ethanolic extracts of C. fistula was tested against Gram positive isolates such as Bacillus cereus, Staphylococcus aureus and Gram-negative isolates such as Salmonella typhi, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa using disc diffusion method and well diffusion method. The 25 mg extract of C. fistula leaves (CF-05) showed more zone of inhibition against Salmonella typhi, i.e., (21mm) and in 50 mg extract of CF-13 fruit showed best zone of inhibition against Salmonella typhi, i.e., 17 mm. Qualitative analysis and antioxidant activity at various concentrations was also measured. The phytochemical analysis showed the presence of alkaloids, carbohydrates, fats, tannins, flavonoids, saponins, terpenoids, and sterols. The antioxidant activity in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity revealed the distinguished antioxidant activity of C. fistula.