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ISSN 2410-955X - An International Biannual Journal
BIOMEDICAL LETTERS
Elucidation of resistance acquisition between various genera of gram-negative bacteria
Fahid Khalid 1, Hina Bukhari 2, Aliya Muzafar 2, Saba Shamim 3, Maria Fayyaz 4*, Aqsa Iqbal 4, Muhammad Iqbal 5

1 Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan
2 Department of Pathology, King Edward Medical University, Lahore, Pakistan
3 Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
4 Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan
5 Department of Botany, University of Okara, Okara, Pakistan

Abstract
The antimicrobial agents have the potential to maintain the selective pressure on bacteria. Bacteria can alter various mechanisms for survival under selective pressure. Thus, the bacteria acquire resistance through conjugation from co-existing bacteria during antimicrobial stress. The study was designed experimentally to investigate the acquired resistance on the basis of time against different genera of gram-negative bacteria. The study was conducted at the Department of Pathology, King Edward Medical University, Mayo Hospital, Lahore, Pakistan. E coli strain was sensitive to Ciprofloxacin and Pseudomonas aeruginosa strain was resistant to Ciprofloxacin. The macro broth dilution technique was used to verify the susceptibility of E. coli and P. aeruginosa. 0.5 MacFarland standard solution of E. coli and P. aeruginosa were mixed and kept at the sub-minimal inhibitory concentration (0.781 µg/ml) of Ciprofloxacin for 28 days. MIC of Ciprofloxacin was measured against E. coli at weekly basis. It was observed that the Ciprofloxacin MIC against E. coli after 7, 14, 21 and 28 days were <01 µg/ml, <01 µg/ml, <04 µg/ml and <25 µg/ml respectively. In conclusion, E. coli acquires resistance from P. aeruginosa under selective pressure of sub-minimal concentration of antibiotic Ciprofloxacin in 28 days.









   



A R T I C L E  I N F O

Received
November 05, 2021
Revised
January 01, 2022
Accepted
February 15, 2022

*Corresponding Author
Maria Fayyaz
E-mail
mariafayyaz58@gmail.com

Keywords
Antimicrobial stress
Resistance
Inhibitory concentration
E. coli
P. aeruginosa






























2022 | Volume 8 | Issue 1