Science Letters

Ameliorative effects of Costus speciosus on biochemical and histopathological changes in alloxan-induced diabetic mice

Ana Triana Maiyah 1, Endang Linirin Widiastuti 1,Sajid Umar 2

1 Department of Biology, Faculty of Mathematics and Sciences, University of Lampung, Indonesia

2 Department of Pathobiology, Faculty of Veterinary & Animal Sciences, Arid Agriculture University, Rawalpindi, Pakistan

Abstract

The present study evaluated the ameliorative effects of Costus speciosus (CS) on biochemical and histopathological changes in alloxanized mice. Diabetes was induced in mice by administration of alloxan monohydrate (0.65mg/100g BW). Root extract of CS (10, 20 and 50 mg/100g BW) and metformin (19.5 mg/100g BW) as the standard drug were administered orally using a gavage to alloxanized mice daily for 14 days.Our study showed that oral administration of CS significantly decreased the blood glucose and total serum cholesterol levels. Moreover, CS restored the altered plasma enzyme (AST, ALT, LDH, ALP and ACP) levels. The reversal in liver and pancreatic histopathology further supports the protective effect of the CS extract towards diabetic damage. Extract of CS is effective in controlling blood glucose in diabetes and protecting liver and pancreatic tissues from diabetic damage. However, further studies are indeed required to prove the safety and efficacy of CS extracts as a potential anti-diabetic agent in clinical practices.

Keywords Alloxan, biochemical changes, Costus speciosus, Diabetes mellitus, histopathology, mice.

Received May 15, 2016 Accepted July 15, 2016 Abstract Published July 30, 2016 Manuscript Published August 15, 2016

*Corresponding author Sajid Umar E-mail s.umar@envt.fr Tel +782316692