Identification and Antimicrobial Susceptibility of Pseudomonas aeruginosa strain PAO1 Isolated from African Catfish Skin Scrapings

Biomedical Sciences | Research article | https://doi.org/10.47262/SL/13.1.132024350

Identification and Antimicrobial Susceptibility of Pseudomonas aeruginosa strain PAO1 Isolated from African Catfish Skin Scrapings

Emmanuel Ugochukwu Anaso¹*, Alhassan Muhammad Salihu²

¹Department of Animal Science, University of Abuja, PMB 117, Giri, Airport Road, Abuja, Nigeria

²Raw Materials Research and Development Council, 17 Aguiyi Ironsi Street, Maitama District, Abuja, Nigeria

Abstract

The current emergence of multidrug-resistant bacteria has posed a significant challenge to public health in Nigeria and Africa as a whole, necessitating studies to evaluate the effectiveness of antibiotics and understand bacterial resistance mechanisms. This study aimed to isolate bacteria from African catfish skin using the spread plate technique and assess antibiotic susceptibility patterns through the diffusion method. The antibiogram results revealed that tarivid exhibited the highest antibacterial activity against a newly isolated Pseudomonas aeruginosa strain PAO1 (96%), followed by ciprofloxacin (78%), chloramphenicol (63%), sparfloxacin (58%), and rufloxacin (56%). However, P. aeruginosa PAO1 demonstrated strong resistance to gentamycin (81%), streptomycin (100%), cloxacillin (100%), cefalexin (100%) and cotrimoxazole (100%). To further elucidate the resistance mechanisms, the findings indicate that while P. aeruginosa infections are highly sensitive to tarivid and ciprofloxacin, their use does not guarantee eradication of all bacterial strains due to the organism's multidrug-resistant nature. Improper administration of these drugs could further contribute to resistance development. This study underscores the importance of targeted antibiotic therapy and the need for careful monitoring of drug use to mitigate the spread of resistant bacterial strains.