Science Letters

In vitro antimicrobial sensitivity pattern of mastitis causing bacterial pathogens isolated from cattle in arid zones of Punjab, Pakistan

Sajid Umar 1*, Fozia Sarwar 2, Muhammad Usman 2, Muhammad Ali A Shah 1, Abdul Ghafar 3, Asif Ali 1, Sajjad Asif 4

1 Department of Pathobiology, PMAS, Arid Agriculture University, Rawalpindi, Pakistan

2 Poultry Research Institute, Rawalpindi, Pakistan

3 NUST Center of Virology &Immunology, Islamabad

4 Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

Abstract

Mastitis is economically most significant disease of lactating animals. Mastitis is a multi-etiological complex disease; however, bacteria are among major pathogens. Bacteria related to mastitis in cattle were identified biochemically and their liability to different antibiotics was judged. Milk samples (n=436) were screened for sub-clinical mastitis by California Mastitis Test. Positive samples (149/436) were cultured on different media and bacterial isolates were identified biochemically. Maximum isolates identified were Staphylococci (44.39%) followed by Streptococci (31.03%), E. coli (12.5%), Bacillus spp. (5.17%), Pseudomonas (3.45%) and Corynebacterium (3.45%). Sensitivity of bacterial isolates was tested for commonly used antibiotics. Gentamicin, enrofloxacin, amoxicillin and cephradine showed best efficacy against the mastitis causing bacteria amongst the antibiotics tested. Ampicillin streptomycin and kanamycin showed moderate sensitivity for mastitis causing bacteria where as least sensitivity was observed to cloxacillin and penicillin. It was concluded that prior to treatment of mastitis, effective antibiotic should be determined through antibiotic sensitivity testing.

Keywords: Cattle, Mastitis, California Mastitis Test, Antibiotic sensitivity, antibiotics.

Received July 15, 2013; Revised August 22, 2013; Accepted September 04, 2013

*Corresponding author: Sajid Umar; Email: Sajncvi@gmail.com